Influence of pH, malic acid and glucose concentrations on malic acid consumption by Saccharomyces cerevisiae
نویسندگان
چکیده
منابع مشابه
Malic acid production by Saccharomyces cerevisiae: engineering of pyruvate carboxylation, oxaloacetate reduction, and malate export.
Malic acid is a potential biomass-derivable "building block" for chemical synthesis. Since wild-type Saccharomyces cerevisiae strains produce only low levels of malate, metabolic engineering is required to achieve efficient malate production with this yeast. A promising pathway for malate production from glucose proceeds via carboxylation of pyruvate, followed by reduction of oxaloacetate to ma...
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متن کاملThe fluorometric determination of malic acid.
A sensitive and specific chemical method for the estimation of malic acid in biological materials has long been desired. The condensation of malic acid with orcinol in the presence of concentrated sulfuric acid to form the highly fluorescent homoumbelliferone (7-hydroxyd-methylcoumarin) was reported years ago (1). Although this reaction is relatively specific for malic acid, the quantitative pr...
متن کاملVariations in mitochondrial membrane potential correlate with malic acid production by natural isolates of Saccharomyces cerevisiae sake strains.
Research on the relationship between mitochondrial membrane potential and fermentation profile is being intensely pursued because of the potential for developing advanced fermentation technologies. In the present study, we isolated naturally occurring strains of yeast from sake mash that produce high levels of malic acid and demonstrate that variations in mitochondrial membrane potential correl...
متن کاملIdentification of malic acid from yeast.
Evidence indicated an accumulation of l-malic acid in the synthetic growth medium of both lysine auxotrophs and wild-type yeast. Malic acid was isolated from growth experiments and was identified by paper and column chromatography, by comparison of infrared spectra with authentic l-malic acid, as well as enzymatically, as a substrate of malic dehydrogenase. Malic acid was accumulated when gluco...
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ژورنال
عنوان ژورنال: Applied Microbiology and Biotechnology
سال: 1995
ISSN: 0175-7598,1432-0614
DOI: 10.1007/s002530050410